17 β-estradiol enhances the outgrowth and survival of neocortical neurons in culture

Roberta Diaz Brinton, Julie Tran, Pam Proffitt, Maria Montoya

Research output: Contribution to journalArticlepeer-review

175 Scopus citations

Abstract

Results of this investigation demonstrate that exposure to 17 β- estradiol differentially and significantly regulates cortical nerve cell outgrowth depending on the cortical region. Parietal and occipital neurons treated with 1 nM 17 β-estradiol showed a greater magnitude of neuronal outgrowth whereas outgrowth of temporal cortex neurons was decreased in the presence of 1 nM 17 β-estradiol. Frontal cortex neurons showed a consistent enhancement of neuronal outgrowth that did not reach statistical significance. The dose response profile for 17 β-estradiol regulation of the macromorphological features exhibited a bimodal dose response relationship whereas the dose response profile for 17 β-estradiol regulation of the micromorphological features exhibited a dose response more characteristic of an inverted V-shaped function. An antagonist to the NMDA receptor antagonist, APS, abolished the growth promoting effect of 17 β-estradiol whereas the nuclear estrogen receptor antagonist ICI 182,780 did not. Lastly, neocortical neurons exposed to 17 β-estradiol exhibited greater viability and survival than control neurons over a two week period. These data indicate that 17 β- estradiol can enhance the growth and viability of select populations of neocortical neurons and that the growth promoting effects of 17 β-estradiol can be blocked by an antagonist to the NMDA glutamate receptor and not by an antagonist to the estrogen nuclear receptor.

Original languageEnglish (US)
Pages (from-to)1339-1351
Number of pages13
JournalNeurochemical Research
Volume22
Issue number11
DOIs
StatePublished - 1997
Externally publishedYes

Keywords

  • Alzheimer's disease
  • Cerebral cortex
  • Estrogen
  • Learning and memory
  • Neurotrophism

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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