1,25-Dihydroxyvitamin D3 receptors in rat kidney cytosol

J. S. Chandler; J. W. Pike; M. R. Haussler

doi:10.1016/0006-291X(79)91933-8

1,25-Dihydroxyvitamin D₃ receptors in rat kidney cytosol

J. S. Chandler, J. W. Pike, M. R. Haussler

Basic Medical Sciences, Phoenix

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

Rat kidney cytosol contains a 3.3 S high affinity binding component for 1,25-dihydroxyvitamin D₃ as detected by DNA-cellulose chromatography and subsequent sucrose gradient analysis. The semipurified aporeceptor demonstrates specificity for 1,25-dihydroxyvitamin D₃ and an apparent dissociation constant for this sterol-hormone of 3.4 × 10^-10M at 25°C. The physicochemical properties of this binding component are in agreement with those observed for the chick intestinal 1,25-dihydroxyvitamin D₃ receptor, suggesting that this component may function as a specific receptor for the hormone in the kidney.

Original language	English (US)
Pages (from-to)	1057-1063
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	90
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(79)91933-8
State	Published - Oct 12 1979

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0006-291X(79)91933-8

Cite this

@article{c67260fc707945488c8a849501bbb423,

title = "1,25-Dihydroxyvitamin D3 receptors in rat kidney cytosol",

abstract = "Rat kidney cytosol contains a 3.3 S high affinity binding component for 1,25-dihydroxyvitamin D3 as detected by DNA-cellulose chromatography and subsequent sucrose gradient analysis. The semipurified aporeceptor demonstrates specificity for 1,25-dihydroxyvitamin D3 and an apparent dissociation constant for this sterol-hormone of 3.4 × 10-10M at 25°C. The physicochemical properties of this binding component are in agreement with those observed for the chick intestinal 1,25-dihydroxyvitamin D3 receptor, suggesting that this component may function as a specific receptor for the hormone in the kidney.",

author = "Chandler, {J. S.} and Pike, {J. W.} and Haussler, {M. R.}",

note = "Funding Information: The authors wish to thank L. Gooz; for her excellent assistance. This research was supported, in part, by an NIH Grant (AM-157811 to MRH. JSC is supported, in part, by GM-01982. JWP is supported, in part, by GM-07239.",

year = "1979",

month = oct,

day = "12",

doi = "10.1016/0006-291X(79)91933-8",

language = "English (US)",

volume = "90",

pages = "1057--1063",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - 1,25-Dihydroxyvitamin D3 receptors in rat kidney cytosol

AU - Chandler, J. S.

AU - Pike, J. W.

AU - Haussler, M. R.

N1 - Funding Information: The authors wish to thank L. Gooz; for her excellent assistance. This research was supported, in part, by an NIH Grant (AM-157811 to MRH. JSC is supported, in part, by GM-01982. JWP is supported, in part, by GM-07239.

PY - 1979/10/12

Y1 - 1979/10/12

N2 - Rat kidney cytosol contains a 3.3 S high affinity binding component for 1,25-dihydroxyvitamin D3 as detected by DNA-cellulose chromatography and subsequent sucrose gradient analysis. The semipurified aporeceptor demonstrates specificity for 1,25-dihydroxyvitamin D3 and an apparent dissociation constant for this sterol-hormone of 3.4 × 10-10M at 25°C. The physicochemical properties of this binding component are in agreement with those observed for the chick intestinal 1,25-dihydroxyvitamin D3 receptor, suggesting that this component may function as a specific receptor for the hormone in the kidney.

AB - Rat kidney cytosol contains a 3.3 S high affinity binding component for 1,25-dihydroxyvitamin D3 as detected by DNA-cellulose chromatography and subsequent sucrose gradient analysis. The semipurified aporeceptor demonstrates specificity for 1,25-dihydroxyvitamin D3 and an apparent dissociation constant for this sterol-hormone of 3.4 × 10-10M at 25°C. The physicochemical properties of this binding component are in agreement with those observed for the chick intestinal 1,25-dihydroxyvitamin D3 receptor, suggesting that this component may function as a specific receptor for the hormone in the kidney.

UR - http://www.scopus.com/inward/record.url?scp=0018612789&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018612789&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(79)91933-8

DO - 10.1016/0006-291X(79)91933-8

M3 - Article

C2 - 508334

AN - SCOPUS:0018612789

SN - 0006-291X

VL - 90

SP - 1057

EP - 1063

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

1,25-Dihydroxyvitamin D₃ receptors in rat kidney cytosol

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this