1,25-dihydroxyvitamin D3 inhibits the clonogenic growth of transformed cells via its receptor

C. A. Haussler; S. L. Marion; J. W. Pike; M. R. Haussler

doi:10.1016/S0006-291X(86)80090-0

1,25-dihydroxyvitamin D₃ inhibits the clonogenic growth of transformed cells via its receptor

C. A. Haussler, S. L. Marion, J. W. Pike, M. R. Haussler

Basic Medical Sciences, Phoenix

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Abstract

Anchorage-independent growth in soft agar is a unique property of transformed cells which is known to be correlated with tumorigenicity. We report here that 1,25-dihydroxyvitamin D₃ suppresses colony formation by a number of cultured cancer cell lines in soft agar in a dose dependent manner with an ID₅₀ of 5-7 × 10^-10M. This effect is also achieved with analogues of 1,25-dihydroxyvitamin D₃ in accordance with their binding affinity for the hormone's receptor. Only cells with 1,25-dihydroxyvitamin D₃ receptor protein are inhibited in their colony formation by vitamin D analogs indicating that the hormone receptor complex may be integrally involved in the in vitro suppression of the anchorage-independent phenotype.

Original language	English (US)
Pages (from-to)	136-143
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	139
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(86)80090-0
State	Published - Aug 29 1986

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "1,25-dihydroxyvitamin D3 inhibits the clonogenic growth of transformed cells via its receptor",

abstract = "Anchorage-independent growth in soft agar is a unique property of transformed cells which is known to be correlated with tumorigenicity. We report here that 1,25-dihydroxyvitamin D3 suppresses colony formation by a number of cultured cancer cell lines in soft agar in a dose dependent manner with an ID50 of 5-7 × 10-10M. This effect is also achieved with analogues of 1,25-dihydroxyvitamin D3 in accordance with their binding affinity for the hormone's receptor. Only cells with 1,25-dihydroxyvitamin D3 receptor protein are inhibited in their colony formation by vitamin D analogs indicating that the hormone receptor complex may be integrally involved in the in vitro suppression of the anchorage-independent phenotype.",

author = "Haussler, {C. A.} and Marion, {S. L.} and Pike, {J. W.} and Haussler, {M. R.}",

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AU - Haussler, C. A.

AU - Marion, S. L.

AU - Pike, J. W.

AU - Haussler, M. R.

N1 - Funding Information: Supported by NIH Grants AM-15781 and AM-33351.

PY - 1986/8/29

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N2 - Anchorage-independent growth in soft agar is a unique property of transformed cells which is known to be correlated with tumorigenicity. We report here that 1,25-dihydroxyvitamin D3 suppresses colony formation by a number of cultured cancer cell lines in soft agar in a dose dependent manner with an ID50 of 5-7 × 10-10M. This effect is also achieved with analogues of 1,25-dihydroxyvitamin D3 in accordance with their binding affinity for the hormone's receptor. Only cells with 1,25-dihydroxyvitamin D3 receptor protein are inhibited in their colony formation by vitamin D analogs indicating that the hormone receptor complex may be integrally involved in the in vitro suppression of the anchorage-independent phenotype.

AB - Anchorage-independent growth in soft agar is a unique property of transformed cells which is known to be correlated with tumorigenicity. We report here that 1,25-dihydroxyvitamin D3 suppresses colony formation by a number of cultured cancer cell lines in soft agar in a dose dependent manner with an ID50 of 5-7 × 10-10M. This effect is also achieved with analogues of 1,25-dihydroxyvitamin D3 in accordance with their binding affinity for the hormone's receptor. Only cells with 1,25-dihydroxyvitamin D3 receptor protein are inhibited in their colony formation by vitamin D analogs indicating that the hormone receptor complex may be integrally involved in the in vitro suppression of the anchorage-independent phenotype.

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1,25-dihydroxyvitamin D₃ inhibits the clonogenic growth of transformed cells via its receptor

Abstract

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