TY - JOUR
T1 - 1,25-Dihydroxyvitamin D3-glycoside
T2 - Identification of a calcinogenic principle of solanum malacoxylon
AU - Haussler, Mark R.
AU - Wasserman, Robert H.
AU - McCain, Toni A.
AU - Peterlik, Meinrad
AU - Bursac, Kristine M.
AU - Hughes, Mark R.
N1 - Funding Information:
This research eras supported by NIH Grants AM 15781 and AM 04b52 . We thank Francis Davie for technical assistance and Dr . Sai Chang of the Department of Internal Medicine, Arizona Medical Cetrter for performing the mesa spectrometry . Please direct all correspondence to Dr . Hauaeler at the University of Arizona.
PY - 1976/5/15
Y1 - 1976/5/15
N2 - The water soluble calcinogenic factor present in the plant Solanum malacoxylon is partially purified by selective extraction and chromatography on silicic acid and then hydrolyzed with a mixed preparation of glycosidases from the sea worm, Charonia lampus. Hydrolysis produces a chloroform soluble factor with biologic characteristics of 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3), the hormonal form of vitamin D. Purification of this factor is accomplished by chromatography on Sephadex LH-20, silicic acid, and Celite columns, yielding 3 μg of active material. During the isolation, bioactivity (as assessed by the ability of fractions to compete with labeled 1,25-(OH)2D3 for binding to a specific intestinal receptor protein) migrates exactly with authentic tritiated 1,25-(OH)2D3. The purified factor has an ultraviolet absorption spectrum identical to that of 1,25-(OH)2D3 and analysis via direct probe mass spectrometry yields a parent molecular ion of m/e 416 and a fragmentation pattern indistinguishable from synthetic 1,25-(OH)2D3 hormone. We therefore conclude that the vitamin D-like principle in Solanum malacoxylon is a sterol-glycoside which contains the 1,25-(OH)2D3 molecule as its active sterol component.
AB - The water soluble calcinogenic factor present in the plant Solanum malacoxylon is partially purified by selective extraction and chromatography on silicic acid and then hydrolyzed with a mixed preparation of glycosidases from the sea worm, Charonia lampus. Hydrolysis produces a chloroform soluble factor with biologic characteristics of 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3), the hormonal form of vitamin D. Purification of this factor is accomplished by chromatography on Sephadex LH-20, silicic acid, and Celite columns, yielding 3 μg of active material. During the isolation, bioactivity (as assessed by the ability of fractions to compete with labeled 1,25-(OH)2D3 for binding to a specific intestinal receptor protein) migrates exactly with authentic tritiated 1,25-(OH)2D3. The purified factor has an ultraviolet absorption spectrum identical to that of 1,25-(OH)2D3 and analysis via direct probe mass spectrometry yields a parent molecular ion of m/e 416 and a fragmentation pattern indistinguishable from synthetic 1,25-(OH)2D3 hormone. We therefore conclude that the vitamin D-like principle in Solanum malacoxylon is a sterol-glycoside which contains the 1,25-(OH)2D3 molecule as its active sterol component.
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U2 - 10.1016/0024-3205(76)90137-5
DO - 10.1016/0024-3205(76)90137-5
M3 - Article
AN - SCOPUS:0017122604
SN - 0024-3205
VL - 18
SP - 1049
EP - 1056
JO - Life Sciences
JF - Life Sciences
IS - 10
ER -