TY - JOUR
T1 - 1-O-Alkyl-2-acyl-sn-glycero-3-phosphocholine
T2 - A novel source of arachidonic acid in neutrophils stimulated by the calcium ionophore A23187
AU - Swendsen, Charles L.
AU - Ellis, J. Marshall
AU - Chilton, Floyd H.
AU - O'Flaherty, Joseph T.
AU - Wykle, Robert L.
N1 - Funding Information:
Acknowledgments. This investigation was supported by Grants HL-26818 and HL-27799 from the National Heart, Lung and Blood Institute; Grant AI-17287 from the National Institute of Allergy and Immunology; and by PHS Grant 5T32 CA-09422 from the National Cancer Institute. We gratefully acknowledge the excellent assistance of Gwen Charles and Tommye Campbell the preparation of this manuscript, and we thank Dr. L. W. Daniel for comments and support.
PY - 1983/5/31
Y1 - 1983/5/31
N2 - Rabbit peritoneal neutrophils incorporated [14C]arachidonic acid into seven molecular species of choline-containing phosphoglycerides. These 2-[14C]arachidonoyl species differed with respect to the alkyl ether or acyl residue bound at the sn-1 position; four of the seven were ether-linked. Stimulation with calcium ionophore A23187 induced a proportionate release of arachidonate from all seven molecular species: 40% of the released arachidonate came from alkyl ether species. Thus, 1-O-alkyl-2-arachidonoyl-sn-glycero-3-phosphocholine (GPC) is a significant source of metabolizable arachidonic acid. Since 1-O-alkyl-2-lyso-GPC is the metabolic precussor of platelet activating factor, these results further interrelate pathways forming arachidonate metabolites and platelet activating factor; they also supply a rationale for the observation that both classes of stimuli form concomitantly during cell activation.
AB - Rabbit peritoneal neutrophils incorporated [14C]arachidonic acid into seven molecular species of choline-containing phosphoglycerides. These 2-[14C]arachidonoyl species differed with respect to the alkyl ether or acyl residue bound at the sn-1 position; four of the seven were ether-linked. Stimulation with calcium ionophore A23187 induced a proportionate release of arachidonate from all seven molecular species: 40% of the released arachidonate came from alkyl ether species. Thus, 1-O-alkyl-2-arachidonoyl-sn-glycero-3-phosphocholine (GPC) is a significant source of metabolizable arachidonic acid. Since 1-O-alkyl-2-lyso-GPC is the metabolic precussor of platelet activating factor, these results further interrelate pathways forming arachidonate metabolites and platelet activating factor; they also supply a rationale for the observation that both classes of stimuli form concomitantly during cell activation.
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U2 - 10.1016/0006-291X(83)90433-3
DO - 10.1016/0006-291X(83)90433-3
M3 - Article
C2 - 6407484
AN - SCOPUS:0020565790
VL - 113
SP - 72
EP - 79
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -