• Friedman, Richard L (PI)

Project: Research project

Grant Details


Pertussis (Whooping cough) is a prolonged, often devastating
infection of the upper respiratory tract caused by Bordetella
pertussis. B. pertussis produces a vast array of potential
virulence factors but which are important in causing disease are
not completely known. The goal of this research is to study one
virulence factor of B. pertussis, the adenylate cyclase in order to
obtain reagents and background information which are necessary
for further studies on the possible role of this toxin in the disease.
Specifically the proposed studies involve: (1) production and purification of the adenylate cyclase for
subsequent characterization studies.
(2) to characterize the purified adenylate cyclase chemically
and to determine possbile subunit structure and function.
(3) to characterize the purified adenylate cyclase biologically
including studies on the effect of the cyclase on
phagocytic/metabolic activities of human and rabbit
phagocytic cells, interaction with respiratory epithelium and
production of monoclonal and affinity purified polyclonal
antibody for immunological and structure-function studies.
(4) and attempt to understand the genetics of the cyclase by
cloning its structural genes.
Effective start/end date4/1/8712/31/90


  • National Institutes of Health


  • Medicine(all)
  • Immunology and Microbiology(all)


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