Transcription profiling by high throughput sequencing of maize B73xMo17 and Mo17xB73 reciprocal F1 hybrids

  • Yi Jia (Contributor)
  • Dan Nettleton (Contributor)
  • Doreen Ware (Contributor)
  • Fusheng Wei (Contributor)
  • Shiran Pasternak (Contributor)
  • Chengzhi Liang (Contributor)
  • Jianwei Zhang (Contributor)
  • L. Fulton (Contributor)
  • Pat Minx (Contributor)
  • Laura Courtney (Contributor)
  • Chad Tomlinson (Contributor)
  • Cindy Strong (Contributor)
  • Kim Delehaunty (Contributor)
  • Catrina Fronick (Contributor)
  • Bill Courtney (Contributor)
  • Eddie Belter (Contributor)
  • Feiyu Du (Contributor)
  • Kyung Kim (Contributor)
  • Marc Cotton (Contributor)
  • Andy Levy (Contributor)
  • Pamela Marchetto (Contributor)
  • Kerri Ochoa (Contributor)
  • Barbara Gillam (Contributor)
  • Weizu Chen (Contributor)
  • Le Yan (Contributor)
  • Jamey Higginbotham (Contributor)
  • Marco Cardenas (Contributor)
  • Jason Waligorski (Contributor)
  • Elizabeth Applebaum (Contributor)
  • Lindsey Phelps (Contributor)
  • Jason Falcone (Contributor)
  • Krishna Kanchi (Contributor)
  • Thynn Thane (Contributor)
  • Adam Scimone (Contributor)
  • Nay Thane (Contributor)
  • Jessica Henke (Contributor)
  • Tom Wang (Contributor)
  • Jessica Ruppert (Contributor)
  • Neha Shah (Contributor)
  • Kelsi Rotter (Contributor)
  • Jennifer Hodges (Contributor)
  • Elizabeth Ingenthron (Contributor)
  • Matt Cordes (Contributor)
  • Sara Kohlberg (Contributor)
  • Jennifer Sgro (Contributor)
  • Brandon Delgado (Contributor)
  • Kelly Mead (Contributor)
  • Asif Chinwalla (Contributor)
  • Shawn Leonard (Contributor)
  • Kevin Crouse (Contributor)
  • Kristi Collura (Contributor)
  • Dave Kudrna (Contributor)
  • Jennifer Currie (Contributor)
  • Ruifeng He (Contributor)
  • Angelina Angelova (Contributor)
  • Shanmugam Rajasekar (Contributor)
  • Teri Mueller (Contributor)
  • Rene Lomeli (Contributor)
  • Gabriel Scara (Contributor)
  • Ara Ko (Contributor)
  • Krista Delaney (Contributor)
  • Marina Wissotski (Contributor)
  • Georgina Lopez (Contributor)
  • David Campos (Contributor)
  • Michele Braidotti (Contributor)
  • Elizabeth Ashley (Contributor)
  • Wolfgang Golser (Contributor)
  • Hye Ran Kim (Contributor)
  • Seunghee Lee (Contributor)
  • Jinke Lin (Contributor)
  • Zeljko Dujmic (Contributor)
  • Woojin Kim (Contributor)
  • Jayson Talag (Contributor)
  • Andrea Zuccolo (Contributor)
  • Chuanzhu Fan (Contributor)
  • Aswathy Sebastian (Contributor)
  • Melissa Kramer (Contributor)
  • Lori Spiegel (Contributor)
  • Lidia Nascimento (Contributor)
  • Theresa Zutavern (Contributor)
  • Beth Miller (Contributor)
  • Claude Ambroise (Contributor)
  • Stephanie Muller (Contributor)
  • William Spooner (Contributor)
  • Apurva Narechania (Contributor)
  • Liya Ren (Contributor)
  • Sharon Wei (Contributor)
  • Sunita Kumari (Contributor)
  • Ben Faga (Contributor)
  • Linda McMahan (Contributor)
  • Peter Van Buren (Contributor)
  • Kai Ying (Contributor)
  • Ananth Kalyanaraman (Contributor)
  • Cristian Chaparro (Contributor)
  • Yan Fu (Contributor)
  • Yujun Han (Contributor)
  • Hye Ran Lee (Contributor)
  • Pinghua Li (Contributor)
  • Sanzhen Liu (Contributor)
  • Zhijie Liu (Contributor)
  • Phillip Sanmiguel (Contributor)
  • John Nguyen (Contributor)
  • Lalit Ponnala (Contributor)
  • Anupma Sharma (Contributor)
  • Cari Soderlund (Contributor)
  • Qi Sun (Contributor)
  • Hao Wang (Contributor)
  • Michael Waterman (Contributor)
  • Richard Westerman (Contributor)
  • Lixing Yang (Contributor)
  • Yeisoo Yu (Contributor)
  • Lifang Zhang (Contributor)
  • Shiguo Zhou (Contributor)
  • Qihui Zhu (Contributor)
  • Jiming Jiang (Contributor)
  • Ning Jiang (Contributor)
  • Srinivas Aluru (Contributor)

Dataset

Description

Heterosis (hybrid vigor) refers to the superior performance of hybrid progeny relative to their parents. Although widely exploited in agriculture, the mechanisms responsible for heterosis are not well understood. As a monoecious organism, a given maize plant can be used as both male and female parents of crosses. Regardless of the cross direction, the maize inbred lines B73 and Mo17 produce hybrids that substantially out-perform their parents. These reciprocal hybrids differ phenotypically from each other despite having identical nuclear genomes. Consistent with these phenotypic observations, 30-50% of genes were differentially expressed between these reciprocal hybrids. An eQTL experiment conducted to better understand the regulation of gene expression in inbred and hybrid lines detected ~4,000 eQTL associations. The majority of these eQTL act in trans to regulate expression of genes on other chromosomes. Surprisingly, many of the trans-eQTL, when heterozygous, differentially regulated transcript accumulation in a manner consistent with gene expression in the hybrid being regulated exclusively by the paternally transmitted allele. The design of the eQTL experiment controlled for cytoplasmic and maternal effects, suggesting that widespread paternal genomic imprinting contributes to the regulation of gene expression in maize hybrids. Keywords: eQTL, parent-of-origin GPL4521 - SAM1.2 (Reciprocal Hybrid Comparison): Six replications of B73xMo17 and Mo17xB73 were grown in growth chambers to tightly control environmental variation. Seeds from each genotype were taken from a single source (ear) for all six replications. Within each replication, genotypes were randomly assigned growth locations. Six healthy seedlings for each genotype and replication were harvested at two weeks of age. For each replication, B73xMo17 and Mo17xB73 were hybridized to the SAM1.2 microarray (GPL4521) using a randomized, alternate dye assignment. GPL3333 - SAM1.1 and GPL3538 - SAM3.0 (eQTL Experiment): Four biological replications of the RIL, B73xRIL, and Mo17xRIL cross-types were planted in growth chambers using seed from a single source for each genotype. Each RIL and its crosses onto B73 and Mo17 were planted using a split-plot design with RIL group (RIL and its cross onto B73 and Mo17) as the whole-plot treatment factor and cross-type (RIL, B73xRIL, and Mo17xRIL) as the split-plot treatment factor. The whole-plot portion of the experiment was designed as a randomized complete block design with four replications carried out on four separate occasions in the same environment. For the split-plot portion of the design, twelve seedlings of each RIL and its crosses were randomized within two adjacent flats in a growth chamber (six healthy seedlings per genotype were randomly chosen and pooled at harvest). For each replication, RIL, B73xRIL, and Mo17xRIL cross-types were hybridized to custom cDNA microarrays using a loop design such that each loop included all pairwise comparisons between the RIL and its crosses with B73 and Mo17. Four biological replications were hybridized to the SAM1.1 (GPL3333) array and two of the four biological replications were hybridized to SAM3.0 (GPL3538). RNA samples were alternately labeled to provide dye balance within each loop and replication. GPL8734 - Gene Expression between two maize reciprocal hybrids Heterosis refers to the enhanced agronomic performance of a hybrid relative to its (usually) inbred parents. We have previously documented widespread differences in gene expression in the B73xMo17 hybrid relative to its inbred parents B73 and Mo17 (Swanson, et al., 2006, PNAS). The reciprocal B73xMo17 and Mo17xB73 hybrids are both highly heterotic, but despite having identical nuclear genomes exhibit statistically significant differences in multiple traits. RNA-seq experiment was conducted to compare the gene expression globally between the two reciprocal hybrids. 1 samples from B73XMo17 and Mo17XB73 RNAs were extracted from a single replication of 14-day-old B73xMo17 and Mo17xB73 seedlings. RNAs were purified using DNaseI treatment followed by cleanup with the RNeasy Plant Mini Kit (Qiagen, Valencia, CA) as per manufacturer instructions. Sequencing library construction was completed using the Illumina mRNA-Seq sample preparation kit. Processed data file 'ZmB73_4a.53_filtered_genes.fasta' and its README file are linked below as supplementary files. The fasta file contains the gene model ID and corresponding sequence generated from maize genome project. This fasta file was used for the following samples: GSM418173, GSM418174, GSM420173, GSM420174, GSM422828, GSM422829.
Date made available2009
PublisherArrayExpress

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