Potential Epigenetic Biomarkers of Obesity Related Insulin Resistance in Human Whole-blood

  • Samantha E. Day (Creator)
  • Richard L. Coletta (Creator)
  • J. Y. Kim (Creator)
  • Luis A. Garcia (Creator)
  • L. E. Campbell (Contributor)
  • Tonya R. Benjamin (Creator)
  • Lori R. Roust (Creator)
  • Elena A. De Filippis (Contributor)
  • Lawrence J Mandarino (Creator)
  • Dawn K Coletta (Creator)



Obesity can increase the risk of complex metabolic diseases, including insulin resistance. Moreover, obesity can be caused by environmental and genetic factors. However, the epigenetic mechanisms of obesity are not well defined. Therefore, the identification of novel epigenetic biomarkers of obesity allows for a more complete understanding of the disease and its underlying insulin resistance. The aim of our study was to identify DNA methylation changes in whole-blood that were strongly associated with obesity and insulin resistance. Whole-blood was obtained from lean (n = 10; BMI = 23.6 ± 0.7 kg/m2) and obese (n = 10; BMI = 34.4 ± 1.3 kg/m2) participants in combination with euglycemic hyperinsulinemic clamps to assess insulin sensitivity. We performed reduced representation bisulfite sequencing on genomic DNA isolated from the blood. We identified 49 differentially methylated cytosines (DMCs; q
Date made available2017

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