Paper-based in vitro tissue chip for delivering programmed mechanical stimuli of local compression and shear flow

  • Kattika Kaarj (Creator)
  • Marianne Madias (Creator)
  • Patarajarin Akarapipad (Contributor)
  • Soohee Cho (Contributor)
  • Jeong-Yeol Yoon (Creator)



Abstract Mechanical stimuli play important roles on the growth, development, and behavior of tissue. A simple and novel paper-based in vitro tissue chip was developed that can deliver two types of mechanical stimuli—local compression and shear flow—in a programmed manner. Rat vascular endothelial cells (RVECs) were patterned on collagen-coated nitrocellulose paper to create a tissue chip. Localized compression and shear flow were introduced by simply tapping and bending the paper chip in a programmed manner, utilizing an inexpensive servo motor controlled by an Arduino microcontroller and powered by batteries. All electrical compartments and a paper-based tissue chip were enclosed in a single 3D-printed enclosure, allowing the whole device to be independently placed within an incubator. This simple device effectively simulated in vivo conditions and induced successful RVEC migration in as early as 5 h. The developed device provides an inexpensive and flexible alternative for delivering mechanical stimuli to other in vitro tissue models. Graphical abstract
Date made available2020

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