MOESM5 of Measuring DNA content in live cells by fluorescence microscopy

  • Cecil J. Gomes (Creator)
  • Michael Harman (Creator)
  • Sara M. Centuori (Creator)
  • Charles William Wolgemuth (Creator)
  • Jesse D. Martinez (Creator)
  • Charles Wolgemuth (Creator)



Additional file 5: Video S1. LCFM was performed on cells labeled with H2B-GFP (green fluorescence), and each cellâ s DNA content was later measured using Hoechst 33342 staining (blue fluorescence), as described within. All images were then concatenated and the ProcessDNA algorithm was employed to quantify DNA content.
Date made available2018

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