MOESM4 of Measuring DNA content in live cells by fluorescence microscopy

  • Cecil J. Gomes (Creator)
  • Michael Harman (Creator)
  • Sara M. Centuori (Creator)
  • Charles William Wolgemuth (Creator)
  • Jesse D. Martinez (Creator)
  • Charles Wolgemuth (Creator)



Additional file 4: Figure S2. Calculating cellular ploidy in live cells using Hoechst 33342. A diagrammatical representation of H2B-GFP labeled cells progressing through mitosis (grey arrows) is shown. DNA ploidy can be calculated for each mitotic cell by summing the nuclear fluorescence of Hoechst 33342 in the nascent daughter cells. A diploid and tetraploid example is illustrated.
Date made available2018

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