All-Reflective Multiphoton Microscope

We present the design and fabrication of a multiphoton microscope that uses reflective elements for beam shaping and steering. This compact all reflective design removes the adverse effects of dispersion on laser pulse broadening as well as chromatic aberration in the focusing of broadband and multicolored laser sources. The design of this system is discussed in detail, including aberrations analysis via ray-tracing simulation and opto-mechanical design. This mirror based all-reflective microscope is characterized using fluorescent microbeads. The performance of the system at multiple wavelengths is investigated along with some potential multiphoton imaging and writing applications.