Additional file 6: Figure S6. of ROCK activity and the Gβγ complex mediate chemotactic migration of mouse bone marrow-derived stromal cells

Inhibitors do not affect BMSC viability or cytotoxicity or induce apoptosis; 24-h serum starvation (SFM) of BMSCs alone or in conjunction with 10 μM Y27632, 5 μM LY294002, 10 μM MPS-Phos, or 0.5 μM Staurosporine. A very low dose of Staurosporine was included as a positive control for apoptosis. Untreated cells (cultured in normal media) were included as an untreated control. SFM set = 1. Graph represents three independent experiments. (Each individual experiment was performed in triplicate.) Data are presented as mean ± standard error of the mean. A P value of less than 0.05 (*) was deemed significant, and a P value of less than 0.01 (**) very significant. BMSC bone marrow-derived stromal cell, SFM serum-free medium. (PNG 360 kb)