Additional file 5 of txci-ATAC-seq: a massive-scale single-cell technique to profile chromatin accessibility

Additional file 5: Table S4. Sequences of Tn5 linker oligos. The ‘N’ bases shown in the Tn5ME-B sequence represent the Tn5 barcodes. Table S5. Tn5 barcode sequences. Column 1 shows the well ID for each well on the iTSM plate. Column 2 shows the sequences of Tn5 barcodes assigned to each well. Column 3 is the 12 numerical labels for the plate columns. Column 4 is the 8 alphabetical labels for the plate rows. This table is provided as a separate file. Table S6. Well IDs of Tn5 barcodes assigned to each sample or species-mixing condition in each experiment. Column 1 shows the figure number for each experiment. Column 2 shows the experimental conditions employed in each figure. Column 3 indicates the species-mixing condition. Column 4 shows the cell source of human samples. Column 5 shows the number of human nuclei loaded to each well. Column 6 shows the cell source of mouse samples. Column 7 shows the number of mouse nuclei loaded to each well. Column 8 indicates the nuclei preparation method (Fresh vs. Frozen). Column 9 is the well ID on the iTSM plate (see Tn5 barcode sequences in Additional file 5: Table S5) assigned to each sample or barnyard experiment. This table is provided as a separate file. Table S7. TruSeq i7 index sequences used for each library in Sample Index PCR. Column 1 shows the index ID. Column 2 shows the oligo sequence. Column 3 indicates the barcode sequence assigned to each library shown in Column 4. Table S8. DNA oligonucleotides used to block barcode swapping. Each row provides the sequence of an oligo used in the barcode swapping blocking tests. The lowercase letters shown in the full SBS primer represent the barcode sequence. For Decoy DNA, the strands A and B were annealed to form a duplex DNA.