Additional file 4: Figure S4. of ROCK activity and the Gβγ complex mediate chemotactic migration of mouse bone marrow-derived stromal cells

Human BMSC chemotactic migration is unaffected by ROCK inhibition but does display the characteristic morphology. a Serum-starved human BMSCs polarise in response to MCP-1; 10 μM Y27632 treatment results in stellate morphology in BMSCs, independent of MCP-1 treatment. Scale bar = 10 μm. b ROCK inhibition does not affect human BMSC migration in response to MCP-1. Immunofluorescent images of representative fields of serum-starved (24 h) BMSCs pre-treated with 10 μM Y27632 and exposed to a gradient of MCP-1 in a 2D assay. Permeabilised serum-starved BMSCs stained with F-actin (red) and α-tubulin (green) and DNA stained with DAPI (blue). Scale bar = 30 μm. c Quantification of b. Graph represents two separate independent experiments (>100 cells evaluated for each separate independent experiment). Data are presented as mean ± standard error of the mean. A P value of less than 0.05 (*) was deemed significant, and a P