Additional file 4: Figure S4. of ROCK activity and the Gβγ complex mediate chemotactic migration of mouse bone marrow-derived stromal cells

  • Caroline M. Ryan (Creator)
  • James A.L. Brown (Creator)
  • Emer Bourke (Creator)
  • Áine M. Prendergast (Creator)
  • Claire Kavanagh (Creator)
  • Zhonglin Liu (Contributor)
  • Peter Owens (Creator)
  • Georgina Shaw (Creator)
  • Walter Kolch (Creator)
  • Timothy O'Brien (Contributor)
  • Frank P. Barry (Creator)

Dataset

Description

Human BMSC chemotactic migration is unaffected by ROCK inhibition but does display the characteristic morphology. a Serum-starved human BMSCs polarise in response to MCP-1; 10 μM Y27632 treatment results in stellate morphology in BMSCs, independent of MCP-1 treatment. Scale bar = 10 μm. b ROCK inhibition does not affect human BMSC migration in response to MCP-1. Immunofluorescent images of representative fields of serum-starved (24 h) BMSCs pre-treated with 10 μM Y27632 and exposed to a gradient of MCP-1 in a 2D assay. Permeabilised serum-starved BMSCs stained with F-actin (red) and α-tubulin (green) and DNA stained with DAPI (blue). Scale bar = 30 μm. c Quantification of b. Graph represents two separate independent experiments (>100 cells evaluated for each separate independent experiment). Data are presented as mean ± standard error of the mean. A P value of less than 0.05 (*) was deemed significant, and a P <0.001 (***) highly significant. 2D two-dimensional, BMSC bone marrow-derived stromal cell, DAPI 4′,6-diamidino-2-phenylindole, MCP-1 monocyte chemoattractant protein 1, ROCK Rho-associated, coiled-coil containing protein kinase. (PNG 1175 kb)
Date made available2015
Publisherfigshare

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