Additional file 3: Figure S3. of Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion

Laminin 521 promotes increased human myogenic cell proliferation. Cells were expanded on laminin 111, laminin 211, laminin 521, or growth factor reduced Matrigel for 5 passages. For proliferation analysis, cells were seeded at a density of approximately 1500 cells per well and incubated for 2Â days. Afterwards, cells were fixed and nuclei counts were performed using Hoechst staining (Life Technologies). Nâ =â 5 technical replicates. Significance was determined by one-way ANOVA with Bonferroni correction. Significance is annotated as less than .0001 (****) with laminin 521 as control.