Additional file 3: Figure S3. of Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion

  • Christopher M. Penton (Creator)
  • Vasudeo Badarinarayana (Contributor)
  • Joy Prisco (Creator)
  • Elaine Powers (Creator)
  • Mark Pincus (Creator)
  • Ronald E. Allen (Creator)
  • Paul R. August (Creator)



Laminin 521 promotes increased human myogenic cell proliferation. Cells were expanded on laminin 111, laminin 211, laminin 521, or growth factor reduced Matrigel for 5 passages. For proliferation analysis, cells were seeded at a density of approximately 1500 cells per well and incubated for 2Â days. Afterwards, cells were fixed and nuclei counts were performed using Hoechst staining (Life Technologies). Nâ =â 5 technical replicates. Significance was determined by one-way ANOVA with Bonferroni correction. Significance is annotated as less than .0001 (****) with laminin 521 as control.
Date made available2016

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