Additional file 2: Figure S2. of ROCK activity and the Gβγ complex mediate chemotactic migration of mouse bone marrow-derived stromal cells

MCP-1 induced significant BMSC migration. a Immunofluorescent images of representative fields of serum-starved (24 h) BMSCs migrating in response to a gradient of MCP-1 in a 2D assay. Permeabilised serum-starved BMSCs immunofluorescently stained with F-actin (red) and α-tubulin (green) and DNA stained with DAPI (blue). Scale bar = 30 μm. b BMSC migration in response to the indicated MCP-1 concentrations in a 3D migration assay. Graph represents three separate independent experiments. Data are presented as mean ± SD. A P value of less than 0.05 (*) was deemed significant. c Representative 3D migration assay results from serum-starved BMSCs exposed to the indicated conditions. 2D two-dimensional, 3D three-dimensional, BMSC bone marrow-derived stromal cell, DAPI 4′,6-diamidino-2-phenylindole, MCP-1 monocyte chemoattractant protein 1, SD standard deviation. (PNG 571 kb)