4Q6X : Structure of phospholipase D Beta1B1i from Sicarius terrosus venom at 2.14 A resolution

  • Daniel M. Lajoie (Contributor)
  • Sue A. Roberts (Contributor)
  • Pamela A. Zobel-Thropp (Contributor)
  • Greta J. Binford (Contributor)
  • Matthew Hj Cordes (Contributor)



Experimental Technique/Method:X-RAY DIFFRACTION
Release Date:2015-03-18
Deposition Date:2014-04-23
Revision Date:2015-04-08#2015-05-20
Molecular Weight:34310.68
Macromolecule Type:Protein
Residue Count:301
Atom Site Count:2241

Venoms of the sicariid spiders contain phospholipase D enzyme toxins that can cause severe dermonecrosis and even death in humans. These enzymes convert sphingolipid and lysolipid substrates to cyclic phosphates by activating a hydroxyl nucleophile present in both classes of lipid. The most medically relevant substrates are thought to be sphingomyelin and/or lysophosphatidylcholine. To better understand the substrate preference of these toxins, we used (31)P NMR to compare the activity of three related but phylogenetically diverse sicariid toxins against a diverse panel of sphingolipid and lysolipid substrates. Two of the three showed significantly faster turnover of sphingolipids over lysolipids, and all three showed a strong preference for positively charged (choline and/or ethanolamine) over neutral (glycerol and serine) headgroups. Strikingly, however, the enzymes vary widely in their preference for choline, the headgroup of both sphingomyelin and lysophosphatidylcholine, versus ethanolamine. An enzyme from Sicarius terrosus showed a strong preference for ethanolamine over choline, whereas two paralogous enzymes from Loxosceles arizonica either preferred choline or showed no significant preference. Intrigued by the novel substrate preference of the Sicarius enzyme, we solved its crystal structure at 2.1 Å resolution. The evolution of variable substrate specificity may help explain the reduced dermonecrotic potential of some natural toxin variants, because mammalian sphingolipids use primarily choline as a positively charged headgroup; it may also be relevant for sicariid predatory behavior, because ethanolamine-containing sphingolipids are common in insect prey.
Date made available2015

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